Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- ABIN2508699 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Leptin (LEP) antibody (Biotin)
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hLeptin. Anti-Human Leptin specific antibody was purified by affinity chromatography and then biotinylated.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Biotin
- Vial size
- 50 μg
- Storage
- -20°C
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Supportive validation
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- antibodies-online (provider)
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- Experimental details
- Western blot analysis of rat brain membranes (lanes 1 and 5), mouse brain membranes (lanes 2 and 6), rat skeletal muscle lysate (lanes 3 and 7) and human SH-SY5Y neuroblastoma cell lysate (lanes 4 and 8): 1- 4. Anti-Na+/Ca2+ Exchanger 2 (NCX-2) antibody (ABIN2511041), (1:200). 5- 8. Anti-Na+/Ca2+ Exchanger 2 (NCX-2) antibody, preincubated with the control peptide antigen. Expression of NCX2 in rat cerebellum Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Na+/Ca2+ Exchanger 2 (NCX-2) antibody (ABIN2511041), (1:100). A. NCX2 staining (red) is expressed in the molecular layer (M) and Purkinje cells (arrows). B. DAPI counterstain (blue) displays the layout of cerebellar layers. C. Merge of A and B demonstrates restriction of NCX2 expression to the molecular layer and Purkinje cells.
Supportive validation
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- antibodies-online (provider)
- Main image
- Experimental details
- Image(s): Immunofluorescence