HPA028666

antibody from Atlas Antibodies

Targeting: SPAG17 CT143, FLJ34497, PF6, RP4-776P7.2

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA028666

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA028666, RRID:AB_10602281

Product name

Anti-SPAG17

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

DLTPLLSFQATDPVNGTVMTTREDKVVIVERKDGT
RIVDHADGTRITTFYQVYEDQIILPDDQETTEGPR
TVTRQVKCMRVESSRYATVIANCEDSSCCATF


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human fallopian tube and prostate tissues using Anti-SPAG17 antibody. Corresponding SPAG17 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human fallopian tube shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human prostate shows low expression as expected.

Sample type

HUMAN