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LearnAF842
antibody from R&D Systems
Targeting: PAEP
GD, GdA, GdF, GdS, MGC138509, MGC142288, PAEG, PEP, PP14
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF842 - Provider product page
- Provider
- R&D Systems
- Product name
- Human PP14/Glycodelin Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human PP14/Glycodelin in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P09466- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Affinity purification of native glycodelin from amniotic fluid for biological investigations and development of a glycodelin ELISA for clinical studies.
Glycosylation failure extends to glycoproteins in gestational diabetes mellitus: evidence from reduced α2-6 sialylation and impaired immunomodulatory activities of pregnancy-related glycodelin-A.
Sørensen S, Myrhøj V, Nguyen TH, Aaslo P, Hansen YB
Protein expression and purification 2017 Feb;130:73-80
Protein expression and purification 2017 Feb;130:73-80
Glycosylation failure extends to glycoproteins in gestational diabetes mellitus: evidence from reduced α2-6 sialylation and impaired immunomodulatory activities of pregnancy-related glycodelin-A.
Lee CL, Chiu PC, Pang PC, Chu IK, Lee KF, Koistinen R, Koistinen H, Seppälä M, Morris HR, Tissot B, Panico M, Dell A, Yeung WS
Diabetes 2011 Mar;60(3):909-17
Diabetes 2011 Mar;60(3):909-17
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human PP14/Glycodelin by Western Blot. Western blot shows lysates of human uterus tissue and human placenta tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human PP14/Glycodelin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF842) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PP14/Glycodelin at approximately 24-27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
