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- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
- Other assay [3]
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- Product number
- STJ70740 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-Complement factor H antibody (Internal) (STJ70740)
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody anti-Complement factor H (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry research applications.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
HLVPDRKKDQYK- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20 on receipt and minimise freeze-thaw cycles.
- Handling
- NA
No comments: Submit comment
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- STJ70740 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- STJ70740 staining (0. 03µg/ml) of Human Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- STJ70740 staining (0. 3µg/ml) of K562 cell lysate (35µg protein in RIPA buffer) Detected by chemiluminescence.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- STJ70740 (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
