F54343

antibody from NSJ Bioreagents

Targeting: CFH ARMD4, ARMS1, FHL1, HF, HF1, HF2, HUS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54343

Provider

NSJ Bioreagents

Product name

CFH Antibody / Complement factor H

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 µl, 0.4 µl

Storage

Aliquot the Complement factor H antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) lung and 2) plasma lysate with Complement factor H antibody. Predicted molecular weight ~139 kDa but may be observed at a higher molecular weight due to glycosylation.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) plasma and 2) kidney lysate with Complement factor H antibody. Predicted molecular weight ~139 kDa but may be observed at a higher molecular weight due to glycosylation.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of fixed and permeabilized human HepG2 cells with Complement factor H antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human hepatocellular carcinoma tissue with Complement factor H antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.