H00165918-M01

antibody from Abnova Corporation

Targeting: RNF168 FLJ35794

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00165918-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00165918-M01, RRID:AB_566136

Product name

RNF168 monoclonal antibody (M01), clone 3E1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant RNF168.

Antigen sequence

MVPNRQKGSPDEYHLRATSSPPDKVLNGQRKNPKD
GNFKRQTHTKHPTPERGSRDKNRQVSLKMQLKQSV
NRRKMPNSTRDHCKVSKSAHSLQPSISQKSVFQMF
QRCTK


Isotype

IgG

Antibody clone number

3E1

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

RNF168 monoclonal antibody (M01), clone 3E1 Western Blot analysis of RNF168 expression in HepG2 ( Cat # L019V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged RNF168 is 0.03 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to RNF168 on HepG2 cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to RNF168 on formalin-fixed paraffin-embedded human prostate cancer. [antibody concentration 1.5 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol