21393-1-AP

antibody from Invitrogen Antibodies

Targeting: RNF168 FLJ35794

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

21393-1-AP

Provider

Invitrogen Antibodies

Product name

RNF168 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: NTGDIQKYL TPKSQFGSAS HSEAVQEVRK DSVSKDIDSS DRKSPTGQDT EIEDMPTLSP QISLGVGEQG ADSSIESPMP WLCACGAEWY HEGNVKTRPS NHGKELCVLS HERPKTRVPY SKETAVMPCG RTESGCAPTS GVTQTNGNNT GETENEESCL LISKEISKRK NQESSFEAVK DPCFSAKRRK VSPESSPDQE ETEINFTQKL IDLEHLLFER HKQEEQDRLL ALQLQKEVDK EQMVPNRQKG SPDEYHLRAT SSPPDKVLNG QRKNPKDGNF KRQTHTKHPT PERGSRDKNR QVSLKMQLKQ SVNRRKMPNS TRDHCKVSKS AHSLQPSISQ KSVFQMFQRC TK (221-571 aa encoded by BC033791)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.49 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 21393-1-AP (RNF168 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 21393-1-AP (RNF168 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 21393-1-AP (RNF168 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 21393-1-AP (RNF168 Antibody) at dilution of 1:500 incubated at 4 degree celsius over night.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using RNF168 antibody 21393-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-RNF168 (IP:21393-1-AP, 4ug; Detection:21393-1-AP 1:500) with HepG2 cells lysate 2400ug.