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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- PA5-40123 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZNF804A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: U87-MG whole cell lysate or nuclear extract.
- Concentration
- 2.44 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ZNF804A was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ZNF804A Polyclonal Antibody (Product # PA5-40123) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ZNF804A Polyclonal Antibody detects ZNF804A protein by western blot analysis. U87-MG whole cell extracts and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ZNF804A Polyclonal Antibody (Product # PA5-40123) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of ZNF804A was performed in U87-MG whole cell extracts using 5 µg of ZNF804A Polyclonal Antibody (Product # PA5-40123). Samples were transferred to a membrane and probed with ZNF804A Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
