R33341

antibody from NSJ Bioreagents

Targeting: AKT3 PKBG, PRKBG, RAC-gamma

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R33341

Provider

NSJ Bioreagents

Product name

AKT3 Antibody

Antibody type

Polyclonal

Antigen

Amino acids CSPTSQIDNIGEEEM were used as the immunogen for this AKT3 antibody. (1)

Description

Antigen affinity purified antibody

Reactivity

Human, Mouse, Rat

Host

Goat

Conjugate

Unconjugated

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

Aliquot and store the AKT3 antibody at -20°C.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of HepG2 [A], Jurkat [B], mouse brain [C] and rat brain [D] lysates with AKT3 antibody at 1ug/ml. Predicted molecular weight: ~56kDa but can be observed from 60~65kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot of HEK293 lysate overexpressing AKT3 probed with AKT3 antibody (mock transfection on right). Predicted molecular weight: ~56kDa but can be observed from 60~65kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human prostate tissue with AKT3 antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human heart tissue with AKT3 antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.