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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
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- Product number
- NB100-1480 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1480, RRID:AB_10002818
- Product name
- Goat Polyclonal AKT3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise an epitope corresponding to aa 119-133 of both isoforms of human AKT3 protein. This antibody does not cross-react with human AKT1/2. Reported variants represent identical protein (NP_859029.1
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references AKT-1, -2, and -3 are expressed in both normal and tumor tissues of the lung, breast, prostate, and colon.
Zinda MJ, Johnson MA, Paul JD, Horn C, Konicek BW, Lu ZH, Sandusky G, Thomas JE, Neubauer BL, Lai MT, Graff JR
Clinical cancer research : an official journal of the American Association for Cancer Research 2001 Aug;7(8):2475-9
Clinical cancer research : an official journal of the American Association for Cancer Research 2001 Aug;7(8):2475-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AKT3 Antibody [NB100-1480] - (1ug/ml) staining of HepG2lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AKT3 Antibody [NB100-1480] - HEK293 overexpressing AKT3 (RC224750) and probed with (mock transfection in second lane)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AKT3 Antibody [NB100-1480] - staining of HepG2 (A), Jurkat (B), Mouse Brain (C) and Rat Brain (D) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: AKT3 Antibody [NB100-1480] - staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: AKT3 Antibody [NB100-1480] - staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
