MAB1463

antibody from R&D Systems

Targeting: AKT3 PKBG, PRKBG, RAC-gamma

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB1463

Provider

R&D Systems

Product name

Human Akt3 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects endogenous human Akt3 in Western blots. Using Western blots, this antibody does not detect recombinant human Akt1 or Akt2.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

Q9Y243


Isotype

IgG

Antibody clone number

199864

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Akt3 by Western Blot. Western blot shows lysates of DU145 human prostate carcinoma cell line and MDA-MB-231 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Human Akt3 Monoclonal Antibody (Catalog # MAB1463) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Akt3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.