GTX25680

antibody from GeneTex

Targeting: INSR CD220

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

GTX25680

Provider

GeneTex

Proper citation

GeneTex Cat#GTX25680, RRID:AB_380648

Product name

IGF1R (phospho Tyr1135 / Tyr1136) / Insulin Receptor (phospho Tyr1150 / Tyr1151) antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Extracts of CHO-T cells over-expressing the human insulin receptor unstimulated (1) or stimulated with 100 nM insulin for 10 min at 37¢XC (2-5) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer overnight at 4¢XC and incubated with the IR/IGF1R [pYpY1162/1163] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 5), the phosphopeptide immunogen (2), the non-phosphorylated peptide corresponding to the phosphopeptide immunogen (3), or a generic phosphotyrosine-containing peptide (4). After washing, the membrane was incubated with goat F(ab)2 anti-rabbit IgG HRP-conjugate and signals were detected. The data show that only the phosphopeptide corresponding to IR/IGF1R [pYpY1162/1163] completely blocks the antibody signal, demonstrating the specificity of the antibody. The data also show the up-regulation of this site upon stimulation with insulin in this cell system.