AM00077PU-N

antibody from OriGene

Targeting: INSR CD220

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

AM00077PU-N

Provider

OriGene

Product name

Insulin Receptor (INSR) (beta chain) (incl. pos. control) mouse monoclonal antibody, clone 9H4, Purified

Antibody type

Monoclonal

Description

Insulin Receptor (INSR) (beta chain) (incl. pos. control) mouse monoclonal antibody, clone 9H4, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

INSR

Isotype

IgG

Antibody clone number

9H4

Vial size

100 µg

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Detection of endogenous InsR: Whole cell extracts of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab InsR-9H4 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 3 min). Lane 1: A431 Lane 2: SW480 Lane 3: SW620 Lane 4: HT29 Lane 5: MCF-7 Lane 6: MDA-MB231 Lane 7: T47D

Validation comment

WB