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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- GTX81012 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX81012, RRID:AB_11162809
- Product name
- GLS antibody, C-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Distinct Regulation of Th17 and Th1 Cell Differentiation by Glutaminase-Dependent Metabolism.
Targeted inhibition of tumor-specific glutaminase diminishes cell-autonomous tumorigenesis.
Johnson MO, Wolf MM, Madden MZ, Andrejeva G, Sugiura A, Contreras DC, Maseda D, Liberti MV, Paz K, Kishton RJ, Johnson ME, de Cubas AA, Wu P, Li G, Zhang Y, Newcomb DC, Wells AD, Restifo NP, Rathmell WK, Locasale JW, Davila ML, Blazar BR, Rathmell JC
Cell 2018 Dec 13;175(7):1780-1795.e19
Cell 2018 Dec 13;175(7):1780-1795.e19
Targeted inhibition of tumor-specific glutaminase diminishes cell-autonomous tumorigenesis.
Xiang Y, Stine ZE, Xia J, Lu Y, O'Connor RS, Altman BJ, Hsieh AL, Gouw AM, Thomas AG, Gao P, Sun L, Song L, Yan B, Slusher BS, Zhuo J, Ooi LL, Lee CG, Mancuso A, McCallion AS, Le A, Milone MC, Rayport S, Felsher DW, Dang CV
The Journal of clinical investigation 2015 Jun;125(6):2293-306
The Journal of clinical investigation 2015 Jun;125(6):2293-306
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of GLS Antibody (C-term) (GTX81012) in mouse liver tissue lysates (35μg/lane). GLS (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of GLS Antibody (C-term) (GTX81012) with Hela cell followed by Alexa FluorºS?489-conjμgated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GLS Antibody (C-term) (GTX81012) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjμgation of the secondary antibody and DAB staining. This data demonstrates the use of the GLS Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GLS Antibody (C-term) (GTX81012) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjμgated goat-anti-rabbit secondary antibodies were used for the analysis.
