Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 15704-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EIF4G1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: IEEYLHLND MKEAVQCVQE LASPSLLFIF VRHGVESTLE RSAIAREHMG QLLHQLLCAG HLSTAQYYQG LYEILELAED MEIDIPHVWL YLAELVTPIL QEGGVPMGEL FREITKPLRP LGKAASLLLE ILGLLCKSMG PKKVGTLWRE AGLSWKEFLP EGQDIGAFVA EQKVEYTLGE ESEAPGQRAL PSEELNRQLE KLLKEGSSNQ RVFDWIEANL SEQQIVSNTL VRALMTAVCY SAIIFETPLR VDVAVLKARA KLLQKYLCDE QKELQALYAL QALVVTLEQP PNLLRMFFDA LYDEDVVKED AFYSWESSKD PAEQQGKGVA LKSVTAFFKW LREAEEESDH N (296-645 aa encoded by BC007788)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.23 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 15704-1-AP (EIF4G1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 15704-1-AP (EIF4G1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using EIF4G1 antibody 15704-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using 15704-1-AP (EIF4G1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Ethacrynic acid treated HepG2 cells using 15704-1-AP (EIF4G1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 15704-1-AP (EIF4G1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 15704-1-AP (EIF4G1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-EIF4G1 (IP:15704-1-AP, 5ug; Detection:15704-1-AP 1:500) with HeLa cells lysate 2000ug.