21369-1-AP

antibody from Invitrogen Antibodies

Targeting: CGN KIAA1319

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

21369-1-AP

Provider

Invitrogen Antibodies

Product name

Cingulin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MAEPRGPVD HGVQIRFITE PVSGAEMGTL RRGGRRPAKD ARASTYGVAV RVQGIAGQPF VVLNSGEKGG DSFGVQIKGA NDQGASGALS SDLELPENPY SQVKGFPAPS QSSTSDEEPG AYWNGKLLRS HSQASLAGPG PVDPSNRSNS MLELAPKVAS PGSTIDTAPL SSVDSLINKF DSQLGGQARG RTGRRTRMLP PEQRKRSKSL DSRLPRDTFE ERERQSTNHW TSSTKYDNHV GTSKQPAQSQ NLSPLSGFSR SRQTQDWVLQ SFEEPRRSAQ DPTMLQFKST PDLLRDQQEA APPGSVDHMK ATIYGILREG SSESETSVRR KVSLVLEKMQ PLVMVSSGST K (1-350 aa encoded by BC146657)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.27 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 21369-1-AP (Cingulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21369-1-AP (Cingulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF-7 cells were subjected to SDS PAGE followed by western blot with 21369-1-AP (Cingulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of A431 cells using 21369-1-AP (Cingulin antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of A431 cells using 21369-1-AP (Cingulin antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-Cingulin (IP:21369-1-AP, 4ug; Detection:21369-1-AP 1:800) with mouse kidney tissue lysate 4800ug.