AM7718a

antibody from Abgent

Targeting: SRC ASV, c-src, SRC1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM7718a

Provider

Abgent

Proper citation

Abgent Cat#AM7718a, RRID:AB_2271089

Product name

SRC Antibody

Antibody type

Monoclonal

Antigen

Other

Description

Purified Mouse Monoclonal Antibody (Mab)

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

Submitted references

Src-stimulated IRTKS phosphorylation enhances cell migration.

Chen G, Li T, Zhang L, Yi M, Chen F, Wang Z, Zhang X
FEBS letters 2011 Oct 3;585(19):2972-8

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

The anti-Src Mab (Cat. #AM7718a) is used in Western blot to detect Src in Jurkat cell lysate.

Primary Ab dilution

1:100~500

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"FG Pancreatic Carcinoma Cell Lines stably expressing vector along (FG-V) the b3 integrin subunit (FG-b3) or a b3 truncation mutant (FG-759x). Src Mab (AM7718a) was diluted 1:500 in 1% BSA/TBST and incubated Overnight at 4 °C. After washing 3x 5 min. with TBST the blots were incubated with 1:5000 Goat anti-mouse or Goat anti-rabbit secondary antibody for 1 hr at Room temperature. The blots were again washed 3x 5 min. with TBST and developed using ECL reagent.Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD."

Primary Ab dilution

1:100~500

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Western blot analysis of lysates from HT29, Jurkat cell line (from left to right), using SRC Antibody(Cat. #AM7718a). AM7718a was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane."

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Fluorescent image of A549 cell stained with SRC Antibody(Cat#AM7718a/SD41014A).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SRC primary antibody (1:25, 1 h at 37!). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37!).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37!).SRC immunoreactivity is localized to Cytoplasm significantly."

Primary Ab dilution

1:10~50