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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX81151 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX81151, RRID:AB_11166127
- Product name
- Src (phospho Tyr416) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Plant galactolipid dLGG suppresses lung metastasis of melanoma through deregulating TNF-α-mediated pulmonary vascular permeability and circulating oxylipin dynamics in mice.
A Novel Plant Sesquiterpene Lactone Derivative, DETD-35, Suppresses BRAFV600E Mutant Melanoma Growth and Overcomes Acquired Vemurafenib Resistance in Mice.
Yang CC, Chang CK, Chang MT, Shyur LF
International journal of cancer 2018 Dec 15;143(12):3248-3261
International journal of cancer 2018 Dec 15;143(12):3248-3261
A Novel Plant Sesquiterpene Lactone Derivative, DETD-35, Suppresses BRAFV600E Mutant Melanoma Growth and Overcomes Acquired Vemurafenib Resistance in Mice.
Feng JH, Nakagawa-Goto K, Lee KH, Shyur LF
Molecular cancer therapeutics 2016 Jun;15(6):1163-76
Molecular cancer therapeutics 2016 Jun;15(6):1163-76
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-SRC-Y419 (GTX81151) in HepG2 cell line lysates (35μg/lane). SRC (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of SRC Antibody (Y419)(GTX81151) with A375 cell followed by Alexa Fluor 488-conjμgated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocarcinoma with Phospho-SRC-Y419.ctrl, which was peroxidase-conjμgated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
