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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- STJ113488 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-Phospho-RPS6-S240/244 antibody (STJ113488)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody anti-Phospho-RPS6-S240/244.
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
NA- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- NA
- Handling
- NA
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Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using Phospho-S6 Ribosomal Protein (RPS6) -S240/244 antibody (STJ113488) at 1:1000 dilution or RPS6 antibody (STJ27852). HeLa cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse pancreas using Phospho-S6 Ribosomal Protein (RPS6) -S240/244 rabbit polyclonal antibody (STJ113488) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded rat brain using Phospho-S6 Ribosomal Protein (RPS6) -S240/244 rabbit polyclonal antibody (STJ113488) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug Phospho-S6 Ribosomal Protein (RPS6) -S240/244 polyclonal antibody (STJ113488). Western blot was performed from the immunoprecipitate using Phospho-RPS6-S240/244 polyclonal antibody (STJ113488) at a dilition of 1:1000. 293T cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
