935701

antibody from BioLegend

Targeting: RPS6 S6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

935701

Provider

BioLegend

Product name

Purified anti-RPS6 Phospho (Ser244)

Antibody type

Monoclonal

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

A18024A

Vial size

25 µg

Concentration

0.50 mg/mL

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysates (15 µg protein) from serum-starved SR cells treated without (-) or with (+) 160 nM PMA for 30 minutes were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL (1:2500 dilution) of purified anti-RPS6 Phospho (Ser244) antibody (clone A18024A). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Equal protein loading was confirmed using a pan RPS6 antibody and Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) used at a 1:25000 dilution (lower). Lane M: molecular weight ladder.

Conjugate

Unconjugated