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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- MAB5436 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Ribosomal Protein S6/RPS6 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. The antibody detects endogenous human, mouse and rat RPS6 in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P62753- Isotype
- IgG
- Antibody clone number
- 522731
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells.
Autour A, C Y Jeng S, D Cawte A, Abdolahzadeh A, Galli A, Panchapakesan SSS, Rueda D, Ryckelynck M, Unrau PJ
Nature communications 2018 Feb 13;9(1):656
Nature communications 2018 Feb 13;9(1):656
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Rat Ribosomal Protein S6 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat Ribosomal Protein S6 Monoclonal Antibody (Catalog # MAB5436) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Ribosomal Protein S6 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Ribosomal Protein S6/RPS6 in MM55K Mouse Cell Line. Ribosomal Protein S6/RPS6 was detected in immersion fixed MM55K mouse kidney cell line using Mouse Anti-Human/Mouse/Rat Ribosomal Protein S6/RPS6 Monoclonal Antibody (Catalog # MAB5436) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
