Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AF4870 - Provider product page
- Provider
- R&D Systems
- Product name
- Human S100A2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human S100A2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) S100A1, -A6, -A7, -A8, -A10, -A11, -A13, -A16, rhS100B and rhS100P is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P29034
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Ca2+/S100 proteins act as upstream regulators of the chaperone-associated ubiquitin ligase CHIP (C terminus of Hsc70-interacting protein).
Shimamoto S, Kubota Y, Yamaguchi F, Tokumitsu H, Kobayashi R
The Journal of biological chemistry 2013 Mar 8;288(10):7158-68
The Journal of biological chemistry 2013 Mar 8;288(10):7158-68
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human S100A2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human S100A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4870) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for S100A2 at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- S100A2 in MDA-MB-231 Human Cell Line. S100A2 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Goat Anti-Human S100A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4870) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to nucleus and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.