PA5-120269

antibody from Invitrogen Antibodies

Targeting: NDUFB3 B12

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-120269

Provider

Invitrogen Antibodies

Product name

NDUFB3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: Mouse heart, Mouse kidney. The target is usually found in the following locations: Matrix side, Mitochondrion inner membrane, Single-pass membrane protein.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.21 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of NDUFB3 in various cell lines using a NDUFB3 Polyclonal antibody (Product # PA5-120269) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 10s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of NDUFB3 in L929 cells using a NDUFB3 Polyclonal antibody (Product # PA5-120269). Blue: DAPI for nuclear staining.