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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-61254 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Alsin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: FQPGLYYSGR QDPTEGDNLP ENHSGSKTPV LLSCSKLGYI SRVTAGKDSY LALVDKNIMG YIASLHELAT TERRFYSKLS D
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Alsin in human cell line U-2 OS shows positivity in intermediate filaments. Samples were probed using an Alsin Polyclonal Antibody (Product # PA5-61254).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Alsin in human cerebellum tissue shows strong cytoplasmic positivity in Purkinje cells. Samples were probed using an Alsin Polyclonal Antibody (Product # PA5-61254).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Alsin was performed on HAP1 WT cell lysate. Antibody-bead conjugate was prepared by adding 2 µg of Alsin Polyclonal Antibody (Product # PA5-61254) with 30 µL of Dynabeads™ Protein A (Product # 10002D) and rocked for ~1 hour at 4 degree celcius. 500 µg of protein was incubated with the antibody-bead conjugate for ~1 hours at 4 degree celcius. Following centrifugation and multiple washes, 4% starting material (SM), 4% unbound fraction (UB) and immunoprecipitated fraction (IP) were processed for immunoblot using a different antibody. Ponceau stained transfer of blot is shown. HC = Heavy chain. Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.
