Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [4]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-614 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-614, RRID:AB_10001315
- Product name
- Rabbit Polyclonal FOXO3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. NB100-614 is specific for human FOXO3a protein.
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Genetic Ablation of FOXO in Mice to Investigate Its Physiological Role.
A TLR/AKT/FoxO3 immune tolerance-like pathway disrupts the repair capacity of oligodendrocyte progenitors.
FOXO protects against age-progressive axonal degeneration.
Exploring MicroRNA Expression Profiles Related to the mTOR Signaling Pathway in Mouse Embryonic Fibroblast Cells Treated with Polyethylenimine.
Oh H, Paik J
Methods in molecular biology (Clifton, N.J.) 2019;1890:239-248
Methods in molecular biology (Clifton, N.J.) 2019;1890:239-248
A TLR/AKT/FoxO3 immune tolerance-like pathway disrupts the repair capacity of oligodendrocyte progenitors.
Srivastava T, Diba P, Dean JM, Banine F, Shaver D, Hagen M, Gong X, Su W, Emery B, Marks DL, Harris EN, Baggenstoss B, Weigel PH, Sherman LS, Back SA
The Journal of clinical investigation 2018 May 1;128(5):2025-2041
The Journal of clinical investigation 2018 May 1;128(5):2025-2041
FOXO protects against age-progressive axonal degeneration.
Hwang I, Oh H, Santo E, Kim DY, Chen JW, Bronson RT, Locasale JW, Na Y, Lee J, Reed S, Toth M, Yu WH, Muller FL, Paik J
Aging cell 2018 Feb;17(1)
Aging cell 2018 Feb;17(1)
Exploring MicroRNA Expression Profiles Related to the mTOR Signaling Pathway in Mouse Embryonic Fibroblast Cells Treated with Polyethylenimine.
Lin CW, Jan MS, Kuo JH
Molecular pharmaceutics 2015 Aug 3;12(8):2858-68
Molecular pharmaceutics 2015 Aug 3;12(8):2858-68
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FOXO3 Antibody [NB100-614] - Detection of Human FOXO3a on HeLa whole cell lysate using NB100-614. FOXO3a was also immunoprecipitated using NB100-613.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FOXO3 Antibody [NB100-614] - Detection of Human and Mouse FOXO3a by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-FOXO3a antibody NB100-614 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FOXO3 Antibody [NB100-614] - FOXO3 Protein expression levels both control group and exercise group for skeletal muscles. This image was submitted via customer Review. *{Mouse WB}*
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FOXO3 Antibody [NB100-614] - Expression of FOXO increases in aging brain. Protein expression of Foxo1, Foxo3, and phospho-T24/32 Foxo1/3 in young (
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: FOXO3 Antibody [NB100-614] - Detection of human FOXO3a by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from MCF-7 cells. Antibodies: Affinity purified rabbit anti-FOXO3a antibody NB100-614 used for IP at 6 ug/mg lysate. FOXO3a was also immunoprecipitated by rabbit anti-FOXO3a antibody NB100-613. For blotting immunoprecipitated FOXO3a, NB100-614 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: FOXO3 Antibody [NB100-614] - Sample: FFPE section of human ovarian carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-FOXO3a used at a dilution of 1:1,000 (1ug/ml). Detection: DAB