- PA5-28228 - Invitrogen Antibodies FDPS antibody

Submitted references Integrating Proteomes for Lung Tissues and Lavage Reveals Pathways That Link Responses in Allergen-Challenged Mice.

Mahood TH, Pascoe CD, Karakach TK, Jha A, Basu S, Ezzati P, Spicer V, Mookherjee N, Halayko AJ
ACS omega 2021 Jan 19;6(2):1171-1189

Regeneration of Cochlear Synapses by Systemic Administration of a Bisphosphonate.

Seist R, Tong M, Landegger LD, Vasilijic S, Hyakusoku H, Katsumi S, McKenna CE, Edge ASB, Stankovic KM
Frontiers in molecular neuroscience 2020;13:87

FDPS promotes glioma growth and macrophage recruitment by regulating CCL20 via Wnt/β-catenin signalling pathway.

Chen Z, Chen G, Zhao H
Journal of cellular and molecular medicine 2020 Aug;24(16):9055-9066

Butyrophilin 3A/CD277-Dependent Activation of Human γδ T Cells: Accessory Cell Capacity of Distinct Leukocyte Populations.

Nerdal PT, Peters C, Oberg HH, Zlatev H, Lettau M, Quabius ES, Sousa S, Gonnermann D, Auriola S, Olive D, Määttä J, Janssen O, Kabelitz D
Journal of immunology (Baltimore, Md. : 1950) 2016 Oct 15;197(8):3059-3068

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of FDPS using 30 µg of A549 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a FDPS polyclonal antibody (Product # PA5-28228) at a dilution of 1:10,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of FDPS was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a FDPS Polyclonal Antibody (Product # PA5-28228) at a dilution of 1:3000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using FDPS Polyclonal Antibody (Product # PA5-28228). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 10% SDS PAGE. FDPS Polyclonal Antibody (Product # PA5-28228) diluted at 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using FDPS Polyclonal Antibody (Product # PA5-28228). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with FDPS Polyclonal Antibody (Product # PA5-28228) diluted at 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FDPS antibody detects FDPS protein by western blot analysis. A. 50 µg rat liver lysate/extract.10% SDS-PAGE. FDPS antibody FDPS Polyclonal Antibody (Product # PA5-28228) dilution: 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of FDPS was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: FDPS Polyclonal Antibody (Product # PA5-28228) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FDPS Polyclonal Antibody detects FDPS protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: FDPS stained by FDPS Polyclonal Antibody (Product # PA5-28228) diluted at 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Paraffin) analysis of FDPS was performed in paraffin-embedded mouse kidney tissue using FDPS Polyclonal Antibody (Product # PA5-28228) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FDPS Polyclonal Antibody detects FDPS protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. FDPS stained by FDPS Polyclonal Antibody (Product # PA5-28228) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FDPS Polyclonal Antibody detects FDPS protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. FDPS stained by FDPS Polyclonal Antibody (Product # PA5-28228) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 1 FDPS is expressed at high levels in glioma tissues. The expression of FDPS in glioma tissue and adjacent non-tumour tissue from same patient was assessed by immunohistochemistry (A), real-time PCR (B) and Western blotting (C). (D) Kaplan-Meier curves showing the overall survival of glioma patients with low versus high FDPS expression ( P = 0.025). Error bars represent the SD, ** P < 0.01 (Scale bars, 50 mum)

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 2 FDPS regulates glioma cell growth. A, The protein expression levels of FDPS in human NHAs and glioma cell lines determined by real-time PCR. B, The protein expression levels of FDPS in human NHAs and glioma cell lines determined by Western blotting. C, A172 and H4 cells with FDPS overexpression were established. The level of FDPS in these established cell lines was verified by Western blotting. D and E, Cell proliferation was examined by MTS in A172 and H4 cells with FDPS overexpression. F, EdU assay of indicated cells with FDPS overexpression. G, Colony formation assays of indicated cells with FDPS overexpression. H, U87 cells with stable expression of shCon/shFDPS were subcutaneous injected into NSG mice. Tumour size was measured every 2 days. I, The weight of tumours formed at day 17th. J, The FDPS expression in tumours was analysed by Western blotting. K, The expression of Ki67 was evaluated by IHC staining. Error bars represent the SD, * P < 0.05; ** P < 0.01; *** P < 0.001 (Scale bars, 50 mum)

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 4 FDPS promotes tumour growth in a macrophage-dependent manner. A, The level of FDPS in GL261 cells was analysed by Western blotting. B and C, Representative immunohistochemistry of F4/80+ cells in sections from glioma tumours obtained from C57BL/6J mice treated with clodronate liposomes or PBS liposomes. D, Volume of GL261 tumours treated as indicated. Error bars represent the SD, * P < 0.05; ** P < 0.01 (Scale bars, 50 mum)

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Western blot validation of lung tissue and BALF proteomics. (A, B) Images of the scanned chemiluminescent film for ARG1, CLC1, and FDPS. Protein bands were normalized to total protein loading (Ponceau S) for quantification. Abbreviations used: BALF: bronchial alveolar lavage fluid, * (Welch's unpaired t -test p.adj

PA5-28228

Antibody data