Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX629479 - Provider product page
- Provider
- GeneTex
- Product name
- IDH1 antibody [GT1521]
- Antibody type
- Monoclonal
- Host
- Mouse
- Vial size
- 100 µl
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cre recombinase antibody [GT10212] detects Cre recombinase protein by Western blot analysis.A. 30 μg HeLa whole cell lysate/extractB. 30 μg whole cell lysate/extract of Cre-tagged protein expressing HeLa cells7.5 % SDS-PAGECre recombinase antibody [GT10212] (GTX629479) dilution: 1:5000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cre recombinase antibody [GT10212] detects Cre recombinase protein by western blot analysis.A. IPTG-induced cell lysate from E. coli expressing Cre recombinant proteins12.5 % SDS-PAGECre recombinase antibody [GT10212] (GTX629479) dilution: 1:10000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cre recombinase antibody immunoprecipitates Cre recombinase protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 £gg whole cell lysate/extract of Cre recombinase protein expressing 293T cells B : Control with 2.5 £gg of pre-immune mouse IgG C : Immunoprecipitation of Cre recombinase protein by 2.6 £gg of Cre recombinase antibody (GTX629479) 7.5% SDS-PAGE The immunoprecipitated Cre recombinase protein was detected by Cre recombinase antibody (GTX629479) diluted at 1 : 5000. EasyBlot anti-mouse IgG (HRP) (GTX221667-01) was used as a secondary reagent.
