Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-32150 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Isocitrate Dehydrogenase 1/IDH1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references AMP-Activated Protein Kinase α2 in Neutrophils Regulates Vascular Repair via Hypoxia-Inducible Factor-1α and a Network of Proteins Affecting Metabolism and Apoptosis.
Abdel Malik R, Zippel N, Frömel T, Heidler J, Zukunft S, Walzog B, Ansari N, Pampaloni F, Wingert S, Rieger MA, Wittig I, Fisslthaler B, Fleming I
Circulation research 2017 Jan 6;120(1):99-109
Circulation research 2017 Jan 6;120(1):99-109
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP2-32150] - Samples: Whole cell lysate (50 ug) from K562, HeLa, 293T, mouse TCMK-1, mouse NIH3T3, and rat C6 cells. Antibodies: Affinity purified rabbit anti-Isocitrate dehydrogenase antibody NBP2-32150 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP2-32150] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-Isocitrate dehydrogenase antibody NBP2-32150 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated Isocitrate dehydrogenase, NBP2-32150 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.