Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [3]
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Validation data
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- Product number
- NBP2-34091 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Isocitrate Dehydrogenase 1/IDH1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Isocitrate Dehydrogenase 1/IDH1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP2-34091] - Simple Western lane view shows a specific band for Isocitrate Dehydrogenase 1/IDH1 in 0.2 mg/ml of HepG2 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP2-34091] - Electropherogram image of the corresponding Simple Western lane view. Isocitrate Dehydrogenase 1/IDH1 antibody was used at 1:25 dilution on HepG2 lysate(s) respectively.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP2-34091] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10, Lane 2: Human cell line HepG2
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP2-34091] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-IDH1 antibody. Remaining relative intensity is presented. Loading control: anti-PPIB.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP2-34091] - Staining of human prostate shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP2-34091] - Staining of human skeletal muscle shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP2-34091] - Staining in human prostate and skeletal muscle tissues using anti-IDH1 antibody. Corresponding IDH1 RNA-seq data are presented for the same tissues.