Antibody data
- Antibody Data
- Antigen structure
- References [27]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 12332-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#12332-1-AP, RRID:AB_2123159
- Product name
- IDH1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated IDH1 antibody (Cat. #12332-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Wild-type IDH1 inhibits the tumor growth through degrading HIF-α in renal cell carcinoma.
Withanolides from dietary tomatillo suppress HT1080 cancer cell growth by targeting mutant IDH1.
Isocitrate dehydrogenase 2 contributes to radiation resistance of oesophageal squamous cell carcinoma via regulating mitochondrial function and ROS/pAKT signalling.
α-Ketoglutarate links p53 to cell fate during tumour suppression.
Insight into novel RNA-binding activities via large-scale analysis of lncRNA-bound proteome and IDH1-bound transcriptome.
Metabolic reprogramming and Notch activity distinguish between non-small cell lung cancer subtypes.
Loss of IDH2 Accelerates Age-related Hearing Loss in Male Mice.
LncRNA IDH1-AS1 links the functions of c-Myc and HIF1α via IDH1 to regulate the Warburg effect.
L-2-Hydroxyglutarate production arises from noncanonical enzyme function at acidic pH.
G6pd Deficiency Does Not Affect the Cytosolic Glutathione or Thioredoxin Antioxidant Defense in Mouse Cochlea.
HIF-1α inhibits IDH-1 expression in osteosarcoma.
PHB Associates with the HIRA Complex to Control an Epigenetic-Metabolic Circuit in Human ESCs.
Curcumol inhibits the proliferation of gastric adenocarcinoma MGC-803 cells via downregulation of IDH1.
Malic enzyme tracers reveal hypoxia-induced switch in adipocyte NADPH pathway usage.
Registered report: IDH mutation impairs histone demethylation and results in a block to cell differentiation.
Loss of 5-hydroxymethylcytosine is linked to gene body hypermethylation in kidney cancer.
Prognostic and diagnostic potential of isocitrate dehydrogenase 1 in esophageal squamous cell carcinoma.
Proteomic profile in Perna viridis after exposed to Prorocentrum lima, a dinoflagellate producing DSP toxins.
Quantitative flux analysis reveals folate-dependent NADPH production.
Up-regulated isocitrate dehydrogenase 1 suppresses proliferation, migration and invasion in osteosarcoma: in vitro and in vivo.
Proteomic investigation of the sinulariolide-treated melanoma cells A375: effects on the cell apoptosis through mitochondrial-related pathway and activation of caspase cascade.
Proteomic analysis of the differences in orbital protein expression in thyroid orbitopathy.
IDH mutation impairs histone demethylation and results in a block to cell differentiation.
An investigation into the cytotoxic effects of 13-acetoxysarcocrassolide from the soft coral Sarcophyton crassocaule on bladder cancer cells.
Liver X receptor agonists augment human islet function through activation of anaplerotic pathways and glycerolipid/free fatty acid cycling.
Leukemic IDH1 and IDH2 mutations result in a hypermethylation phenotype, disrupt TET2 function, and impair hematopoietic differentiation.
Cancer-associated IDH1 mutations produce 2-hydroxyglutarate.
Chen S, Wang Y, Xiong Y, Peng T, Lu M, Zhang L, Guo Z
International journal of biological sciences 2021;17(5):1250-1262
International journal of biological sciences 2021;17(5):1250-1262
Withanolides from dietary tomatillo suppress HT1080 cancer cell growth by targeting mutant IDH1.
Yang Y, Xiang K, Sun D, Zheng M, Song Z, Li M, Wang X, Li H, Chen L
Bioorganic & medicinal chemistry 2021 Apr 15;36:116095
Bioorganic & medicinal chemistry 2021 Apr 15;36:116095
Isocitrate dehydrogenase 2 contributes to radiation resistance of oesophageal squamous cell carcinoma via regulating mitochondrial function and ROS/pAKT signalling.
Chen X, Zhuo S, Xu W, Chen X, Huang D, Sun X, Cheng Y
British journal of cancer 2020 Jul;123(1):126-136
British journal of cancer 2020 Jul;123(1):126-136
α-Ketoglutarate links p53 to cell fate during tumour suppression.
Morris JP 4th, Yashinskie JJ, Koche R, Chandwani R, Tian S, Chen CC, Baslan T, Marinkovic ZS, Sánchez-Rivera FJ, Leach SD, Carmona-Fontaine C, Thompson CB, Finley LWS, Lowe SW
Nature 2019 Sep;573(7775):595-599
Nature 2019 Sep;573(7775):595-599
Insight into novel RNA-binding activities via large-scale analysis of lncRNA-bound proteome and IDH1-bound transcriptome.
Liu L, Li T, Song G, He Q, Yin Y, Lu JY, Bi X, Wang K, Luo S, Chen YS, Yang Y, Sun BF, Yang YG, Wu J, Zhu H, Shen X
Nucleic acids research 2019 Mar 18;47(5):2244-2262
Nucleic acids research 2019 Mar 18;47(5):2244-2262
Metabolic reprogramming and Notch activity distinguish between non-small cell lung cancer subtypes.
Sellers K, Allen TD, Bousamra M 2nd, Tan J, Méndez-Lucas A, Lin W, Bah N, Chernyavskaya Y, MacRae JI, Higashi RM, Lane AN, Fan TW, Yuneva MO
British journal of cancer 2019 Jul;121(1):51-64
British journal of cancer 2019 Jul;121(1):51-64
Loss of IDH2 Accelerates Age-related Hearing Loss in Male Mice.
White K, Kim MJ, Han C, Park HJ, Ding D, Boyd K, Walker L, Linser P, Meneses Z, Slade C, Hirst J, Santostefano K, Terada N, Miyakawa T, Tanokura M, Salvi R, Someya S
Scientific reports 2018 Mar 22;8(1):5039
Scientific reports 2018 Mar 22;8(1):5039
LncRNA IDH1-AS1 links the functions of c-Myc and HIF1α via IDH1 to regulate the Warburg effect.
Xiang S, Gu H, Jin L, Thorne RF, Zhang XD, Wu M
Proceedings of the National Academy of Sciences of the United States of America 2018 Feb 13;115(7):E1465-E1474
Proceedings of the National Academy of Sciences of the United States of America 2018 Feb 13;115(7):E1465-E1474
L-2-Hydroxyglutarate production arises from noncanonical enzyme function at acidic pH.
Intlekofer AM, Wang B, Liu H, Shah H, Carmona-Fontaine C, Rustenburg AS, Salah S, Gunner MR, Chodera JD, Cross JR, Thompson CB
Nature chemical biology 2017 May;13(5):494-500
Nature chemical biology 2017 May;13(5):494-500
G6pd Deficiency Does Not Affect the Cytosolic Glutathione or Thioredoxin Antioxidant Defense in Mouse Cochlea.
White K, Kim MJ, Ding D, Han C, Park HJ, Meneses Z, Tanokura M, Linser P, Salvi R, Someya S
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Jun 7;37(23):5770-5781
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Jun 7;37(23):5770-5781
HIF-1α inhibits IDH-1 expression in osteosarcoma.
Liu DC, Zheng X, Zho Y, Yi WR, Li ZH, Hu X, Yu AX
Oncology reports 2017 Jul;38(1):336-342
Oncology reports 2017 Jul;38(1):336-342
PHB Associates with the HIRA Complex to Control an Epigenetic-Metabolic Circuit in Human ESCs.
Zhu Z, Li C, Zeng Y, Ding J, Qu Z, Gu J, Ge L, Tang F, Huang X, Zhou C, Wang P, Zheng D, Jin Y
Cell stem cell 2017 Feb 2;20(2):274-289.e7
Cell stem cell 2017 Feb 2;20(2):274-289.e7
Curcumol inhibits the proliferation of gastric adenocarcinoma MGC-803 cells via downregulation of IDH1.
Zang S, Tang Q, Dong F, Liu H, Li L, Guo F, Pan X, Lin H, Zeng W, Cai Z, Zhong Q, Zang N, Zang L
Oncology reports 2017 Dec;38(6):3583-3591
Oncology reports 2017 Dec;38(6):3583-3591
Malic enzyme tracers reveal hypoxia-induced switch in adipocyte NADPH pathway usage.
Liu L, Shah S, Fan J, Park JO, Wellen KE, Rabinowitz JD
Nature chemical biology 2016 May;12(5):345-52
Nature chemical biology 2016 May;12(5):345-52
Registered report: IDH mutation impairs histone demethylation and results in a block to cell differentiation.
Richarson AD, Scott DA, Zagnitko O, Aza-Blanc P, Chang CC, Russler-Germain DA, Reproducibility Project: Cancer Biology.
eLife 2016 Mar 11;5:e10860
eLife 2016 Mar 11;5:e10860
Loss of 5-hydroxymethylcytosine is linked to gene body hypermethylation in kidney cancer.
Chen K, Zhang J, Guo Z, Ma Q, Xu Z, Zhou Y, Xu Z, Li Z, Liu Y, Ye X, Li X, Yuan B, Ke Y, He C, Zhou L, Liu J, Ci W
Cell research 2016 Jan;26(1):103-18
Cell research 2016 Jan;26(1):103-18
Prognostic and diagnostic potential of isocitrate dehydrogenase 1 in esophageal squamous cell carcinoma.
Chen X, Li Q, Wang C, Xu W, Han L, Liu Y, Liu B, Guan S, Tan B, Wang J, Wang N, Song Q, Jia Y, Wang J, Zhao L, Cheng Y
Oncotarget 2016 Dec 27;7(52):86148-86160
Oncotarget 2016 Dec 27;7(52):86148-86160
Proteomic profile in Perna viridis after exposed to Prorocentrum lima, a dinoflagellate producing DSP toxins.
Huang L, Zou Y, Weng HW, Li HY, Liu JS, Yang WD
Environmental pollution (Barking, Essex : 1987) 2015 Jan;196:350-7
Environmental pollution (Barking, Essex : 1987) 2015 Jan;196:350-7
Quantitative flux analysis reveals folate-dependent NADPH production.
Fan J, Ye J, Kamphorst JJ, Shlomi T, Thompson CB, Rabinowitz JD
Nature 2014 Jun 12;510(7504):298-302
Nature 2014 Jun 12;510(7504):298-302
Up-regulated isocitrate dehydrogenase 1 suppresses proliferation, migration and invasion in osteosarcoma: in vitro and in vivo.
Hu X, Liu Y, Qin C, Pan Z, Luo J, Yu A, Cheng Z
Cancer letters 2014 Apr 28;346(1):114-21
Cancer letters 2014 Apr 28;346(1):114-21
Proteomic investigation of the sinulariolide-treated melanoma cells A375: effects on the cell apoptosis through mitochondrial-related pathway and activation of caspase cascade.
Li HH, Su JH, Chiu CC, Lin JJ, Yang ZY, Hwang WI, Chen YK, Lo YH, Wu YJ
Marine drugs 2013 Jul 22;11(7):2625-42
Marine drugs 2013 Jul 22;11(7):2625-42
Proteomic analysis of the differences in orbital protein expression in thyroid orbitopathy.
Cheng KC, Huang HH, Hung CT, Chen CC, Wu WC, Suen JL, Chen KJ, Wu YJ, Chang CH
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie 2013 Dec;251(12):2777-87
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie 2013 Dec;251(12):2777-87
IDH mutation impairs histone demethylation and results in a block to cell differentiation.
Lu C, Ward PS, Kapoor GS, Rohle D, Turcan S, Abdel-Wahab O, Edwards CR, Khanin R, Figueroa ME, Melnick A, Wellen KE, O'Rourke DM, Berger SL, Chan TA, Levine RL, Mellinghoff IK, Thompson CB
Nature 2012 Feb 15;483(7390):474-8
Nature 2012 Feb 15;483(7390):474-8
An investigation into the cytotoxic effects of 13-acetoxysarcocrassolide from the soft coral Sarcophyton crassocaule on bladder cancer cells.
Su CC, Su JH, Lin JJ, Chen CC, Hwang WI, Huang HH, Wu YJ
Marine drugs 2011 Dec;9(12):2622-42
Marine drugs 2011 Dec;9(12):2622-42
Liver X receptor agonists augment human islet function through activation of anaplerotic pathways and glycerolipid/free fatty acid cycling.
Ogihara T, Chuang JC, Vestermark GL, Garmey JC, Ketchum RJ, Huang X, Brayman KL, Thorner MO, Repa JJ, Mirmira RG, Evans-Molina C
The Journal of biological chemistry 2010 Feb 19;285(8):5392-404
The Journal of biological chemistry 2010 Feb 19;285(8):5392-404
Leukemic IDH1 and IDH2 mutations result in a hypermethylation phenotype, disrupt TET2 function, and impair hematopoietic differentiation.
Figueroa ME, Abdel-Wahab O, Lu C, Ward PS, Patel J, Shih A, Li Y, Bhagwat N, Vasanthakumar A, Fernandez HF, Tallman MS, Sun Z, Wolniak K, Peeters JK, Liu W, Choe SE, Fantin VR, Paietta E, Löwenberg B, Licht JD, Godley LA, Delwel R, Valk PJ, Thompson CB, Levine RL, Melnick A
Cancer cell 2010 Dec 14;18(6):553-67
Cancer cell 2010 Dec 14;18(6):553-67
Cancer-associated IDH1 mutations produce 2-hydroxyglutarate.
Dang L, White DW, Gross S, Bennett BD, Bittinger MA, Driggers EM, Fantin VR, Jang HG, Jin S, Keenan MC, Marks KM, Prins RM, Ward PS, Yen KE, Liau LM, Rabinowitz JD, Cantley LC, Thompson CB, Vander Heiden MG, Su SM
Nature 2009 Dec 10;462(7274):739-44
Nature 2009 Dec 10;462(7274):739-44
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 12332-1-AP(IDH1 antibody) at dilution of 1:600
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The IDH1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human IDH1. This antibody recognizes human,mouse,rat antigen. The IDH1 antibody has been validated for the following applications: ELISA, IHC, IP, IF, WB analysis.
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-IDH1 (IP:12332-1-AP, 3ug; Detection:12332-1-AP 1:600) with MCF-7 cells lysate 2500ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human kidney using 12332-1-AP(IDH1 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human kidney using 12332-1-AP(IDH1 antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue