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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Western blot [1]
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- Product number
- 655002 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#655002, RRID:AB_2561892
- Product name
- Purified anti-PTEN
- Antibody type
- Monoclonal
- Antigen
- Partial human PTEN recombinant protein (303-403aa)
- Description
- PTEN was discovered as a tumor suppressor with lipid and protein phosphatase activity. Heterozygous loss of PTEN in mice contribute to the development of a variety of types of cancer, suggesting that insufficient PTEN activity results in increased cancer susceptibility. PTEN negatively regulates the PI3K/AKT pathway by dephosphorylating the second messenger phosphoinositide 3,4,5-triphosphate (PIP3) produced by phosphoinositide 3-kinase (PI3K), which results in inhibition of abnormal cell proliferation. Despite the hydrolysis function of PIP3, the nuclear population of PTEN possesses greater tumor suppressive activities, regulates gene expression, differentiation, and cell cycle progression.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 4C11A11
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- MCF7 cells were stained with purified anti-PTEN (4C11A11) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green) antibody. Nuclei were stained with DAPI (blue).
- Conjugate
- Unconjugated
