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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX115629 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX115629, RRID:AB_11167260
- Product name
- HELIOS antibody [N3C3]
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Quantitative Multiplexed Imaging Analysis Reveals a Strong Association between Immunogen-Specific B Cell Responses and Tonsillar Germinal Center Immune Dynamics in Children after Influenza Vaccination.
Clinical significance of expanded Foxp3⁺ Helios⁻ regulatory T cells in patients with non-small cell lung cancer.
Amodio D, Cotugno N, Macchiarulo G, Rocca S, Dimopoulos Y, Castrucci MR, De Vito R, Tucci FM, McDermott AB, Narpala S, Rossi P, Koup RA, Palma P, Petrovas C
Journal of immunology (Baltimore, Md. : 1950) 2018 Jan 15;200(2):538-550
Journal of immunology (Baltimore, Md. : 1950) 2018 Jan 15;200(2):538-550
Clinical significance of expanded Foxp3⁺ Helios⁻ regulatory T cells in patients with non-small cell lung cancer.
Muto S, Owada Y, Inoue T, Watanabe Y, Yamaura T, Fukuhara M, Okabe N, Matsumura Y, Hasegawa T, Osugi J, Hoshino M, Higuchi M, Suzuki H, Gotoh M
International journal of oncology 2015 Dec;47(6):2082-90
International journal of oncology 2015 Dec;47(6):2082-90
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE GTX115629 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HELIOS antibody detects HELIOS protein by western blot analysis. Jurkat whole cell extracts and nuclear extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with HELIOS antibody (GTX115629) diluted by 1:500 and developed with Trident femto Western HRP Substrate (GTX14698). The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using HELIOS(GTX115629) antibody at 1:250 dilution.
