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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunoprecipitation [2]
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- Product number
- AF73091 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Helios Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Helios in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q9UKS7- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Immunoprecipitation of Human Helios. Helios was immunoprecipitated from 400 µg of Jurkat human acute T cell leukemia cell line and MOLT-4 human acute lymphoblastic leukemia cell line lysates using 4 µg of Sheep Anti-Human Helios Antigen Affinity-purified Polyclonal Antibody (Catalog # AF73091) coated on 4 wells of a 96 well plate (Corning Costar EIA/RIA). Jurkat or MOLT-4 lysates were added to the wells and incubated for 2 hours at room temperature. Immunoprecipitated Helios was detected by Western blot under reducing conditions using 0.1 µg/mL Mouse Anti-Human Helios Monoclonal Antibody (Catalog # MAB73091) and Immunoblot Buffer Group 1. View our recommended buffer recipes for Immunoprecipitation.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Immunoprecipitation of Human Helios. Helios was immunoprecipitated from 400 µg of Jurkat human acute T cell leukemia cell line and MOLT-4 human acute lymphoblastic leukemia cell line lysates using 4 µg of Sheep Anti-Human Helios Antigen Affinity-purified Polyclonal Antibody (Catalog # AF73091) coated on 4 wells of a 96 well plate (Corning Costar EIA/RIA). Jurkat lysates, MOLT-4 lysates, or control buffer were added to the wells and incubated for 2 hours at room temperature. Immunoprecipitated Helios was detected by Western blot under reducing conditions using 0.1 µg/mL Sheep Anti-Human Helios Antigen Affinity-purified Polyclonal Antibody (Catalog # AF73091) and Immunoblot Buffer Group 1. View our recommended buffer recipes for Immunoprecipitation.
