13554-1-AP

antibody from Invitrogen Antibodies

Targeting: IKZF2 Helios, ZNFN1A2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

13554-1-AP

Provider

Invitrogen Antibodies

Product name

IKZF2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: NNISLVPFE RPAVIEKLTG NMGKRKSSTP QKFVGEKLMR FSYPDIHFDM NLTYEKEAEL MQSHMMDQAI NNAITYLGAE ALHPLMQHPP STIAEVAPVI SSAYSQVYHP NRIERPISRE TADSHENNMD GPISLIRPKS RPQEREASPS NSCLDSTDSE SSHDDHQSYQ GHPALNPKRK QSPAYMKEDV KALDTTKAPK GSLKDIYKVF NGEGEQIRAF KCEHCRVLFL DHVMYTIHMG CHGYRDPLEC NICGYRSQDR YEFSSHIVRG EHTFH (227-526 aa encoded by BC028936)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.32 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 13554-1-AP ( IKZF2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human placenta tissue were subjected to SDS PAGE followed by western blot with 13554-1-AP (IKZF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA) fixed HeLa cells using 13554-1-AP (IKZF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lymphoma using 13554-1-AP (IKZF2 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lymphoma using 13554-1-AP (IKZF2 antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-IKZF2 (IP:13554-1-AP, 4ug; Detection:13554-1-AP 1:300) with Jurkat cells lysate 2400ug.