137201

antibody from BioLegend

Targeting: IKZF2 Helios, ZNFN1A2

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

137201

Provider

BioLegend

Proper citation

BioLegend Cat#137201, RRID:AB_10933086

Product name

Purified anti-mouse/human Helios

Antibody type

Monoclonal

Antigen

Helios peptide

Description

Helios is a member of the Ikaros family of zinc finger transcription factors.  It contains a C-terminal region composed of 2 zinc-finger domains that mediate dimerization between the family members.  Helios was originally cloned from a mouse thymoma line. It is mainly expressed in peripheral T cells and thymocytes.  It is found at high levels in a subpopulation of regulatory T cells.  Helios plays an important role in T cell development and homeostasis.  Overexpression of Helios profoundly alters αβ T cell differentiation and activation.  It has been determined that silencing of Helios in B cells is critical for maintaining normal B cell function.  Helios is involved in tumor immunity.

Reactivity

Human, Mouse

Host

Hamster

Conjugate

Unconjugated

Isotype

IgG

Vial size

25 µg

Concentration

0.5 mg/ml

Storage

The antibody solution should be stored undiluted at 4°C.

References

Submitted references

Efficient presentation of myelin oligodendrocyte glycoprotein peptides but not protein by astrocytes from HLA-DR2 and HLA-DR4 transgenic mice.

Kort JJ, Kawamura K, Fugger L, Weissert R, Forsthuber TG
Journal of neuroimmunology 2006 Apr;173(1-2):23-34

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

C57BL/6 splenocytes surface stained with CD4-APC (GK1.5), fixed and permeabilized with BioLegend's FOXP3 Fix/Perm Buffer Set (cat# 421403), and then intracellularly stained with Helios-PE (clone 22F6).

Conjugate

Unconjugated