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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 25930-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IQGAP3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: LINEALDKG SPEKTLSALL LPAAGLDDVS LPVAPRYHLL LVAAKRQKAQ VTGDPGAVLW LEEIRQGVVR ANQDTNTAQR MALGVAAINQ AIKEGKAAQT ERVLRNPAVA LRGVVPDCAN GYQRALESAM AKKQCPADTA FWVQHDMKDG TAYYFHLQTF QGIWEQPPGC PLNTSHLTRE EIQSAVTKVT AAYDRQQLWK ANVGFVIQLQ ARLRGFLVRQ KFAEHSHFLR TWLPAVIKIQ AHWRGYRQRK IYLEWLQYFK ANLDAIIKIQ AWARMWAARR QYLRRLHYFQ KNVNSIVKIQ AFFRARKAQD DYRILVHAPH PPLSVVRRFA HLLNQSQQDF LAEAELLKLQ EE (530-880 aa encoded by BC131536)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 25930-1-AP (IQGAP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF-7 cells were subjected to SDS PAGE followed by western blot with 25930-1-AP (IQGAP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 25930-1-AP (IQGAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human stomach cancer tissue slide using 25930-1-AP (IQGAP3 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human stomach cancer tissue slide using 25930-1-AP (IQGAP3 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
