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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- PAB10063 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10063, RRID:AB_1714309
- Product name
- SMARCAL1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of SMARCAL1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Mutant chromatin remodeling protein SMARCAL1 causes Schimke immuno-osseous dysplasia.
Cloning and characterization of HARP/SMARCAL1: a prokaryotic HepA-related SNF2 helicase protein from human and mouse.
Boerkoel CF, Takashima H, John J, Yan J, Stankiewicz P, Rosenbarker L, André JL, Bogdanovic R, Burguet A, Cockfield S, Cordeiro I, Fründ S, Illies F, Joseph M, Kaitila I, Lama G, Loirat C, McLeod DR, Milford DV, Petty EM, Rodrigo F, Saraiva JM, Schmidt B, Smith GC, Spranger J, Stein A, Thiele H, Tizard J, Weksberg R, Lupski JR, Stockton DW
Nature genetics 2002 Feb;30(2):215-20
Nature genetics 2002 Feb;30(2):215-20
Cloning and characterization of HARP/SMARCAL1: a prokaryotic HepA-related SNF2 helicase protein from human and mouse.
Coleman MA, Eisen JA, Mohrenweiser HW
Genomics 2000 May 1;65(3):274-82
Genomics 2000 May 1;65(3):274-82
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using SMARCAL1 polyclonal antibody (Cat # PAB10063) shows detection of a band ~106 KDa band (arrowhead) corresponding to SMARCAL1 in human derived cultured cell lysates (HeLa nuclear extract at lane 1, HeLa at lane 2, A-431 at lane 3, Jurkat at lane 4, and 293 at lane 5).Approximately 5 ug of each lysates was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of SMARCAL1 polyclonal antibody.Signal was detected using standard techniques.SMARCAL1 is the band seen between the 97 and 116kD markers.
