693402

antibody from BioLegend

Targeting: HDAC5 FLJ90614, KIAA0600, NY-CO-9

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

693402

Provider

BioLegend

Proper citation

BioLegend Cat#693402, RRID:AB_2632835

Product name

Purified anti-HDAC5

Antibody type

Monoclonal

Description

Histone deacetylases (HDACs) are a family of enzymes capable of regulating acetylation level of histones and non-histone proteins. HDACs have been classified into four classes (class I, II, III, and IV) based on their homology with yeast HDACs. HDAC5 is a member of class IIa HDACs, which contain N-terminal extensions with 14-3-3 binding sites. Phosphorylation of HDAC5 on Ser259 and Ser498 leads to association with 14-3-3 proteins and export from the nucleus. HDAC5 has been suggested to participate in various cellular activities, such as cell proliferation, cell cycle progression, apoptosis, neuronal repair, synoviocyte activation, and myoblast differentiation. Aberrant expression of HDAC5 has been shown to be associated with tumor progression and poor prognosis in cancer patients.

Reactivity

Human, Mouse

Host

Rat

Conjugate

Unconjugated

Antibody clone number

W15202A

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysate from Jurkat cells (lane 1, 15 µg), 293T (lane 2, 15 µg) and NIH3T3 (lane 3, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-HDAC5 antibody (clone W15202A, 2 µg/ml). Proteins were visualized using an HRP goat anti-mouse IgG antibody and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.

Conjugate

Unconjugated