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16166-1-AP

antibody from Invitrogen Antibodies

Targeting: HDAC5 FLJ90614, KIAA0600, NY-CO-9

Provider product page for 16166-1-AP

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [0]
Comments [0]
Validations
Western blot [6]
Immunocytochemistry [2]
Immunohistochemistry [6]

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Validation data

Product number: 16166-1-AP - Provider product page
Provider: Invitrogen Antibodies
Product name: HDAC5 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: This antibody only binds HDAC5. It does not cross-react with other HDACs.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 150 µL
Concentration: 0.19 mg/mL
Storage: -20°C

HDAC5 protein structure - 16166-1-AP shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 16166-1-AP (HDAC5-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HEK-293 cells were subjected to SDS PAGE followed by western blot with 16166-1-AP (HDAC5-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Human brain tissue were subjected to SDS PAGE followed by western blot with 16166-1-AP (HDAC5-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HEK-293 cells were subjected to SDS PAGE followed by western blot with 16166-1-AP (HDAC5-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HEK-293 cells were subjected to SDS PAGE followed by western blot with 16166-1-AP (HDAC5-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: MCF-7 cells were subjected to SDS PAGE followed by western blot with 16166-1-AP (HDAC5-specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 16166-1-AP (HDAC5-specific Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 16166-1-AP (HDAC5-specific Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human heart tissue slide using 16166-1-AP (HDAC5-specific Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human heart tissue slide using 16166-1-AP (HDAC5-specific Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human brain using 16166-1-AP (HDAC5-specific antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human brain using 16166-1-AP (HDAC5-specific antibody) at dilution of 1:50 (under 40x lens).