PA1-41117

antibody from Invitrogen Antibodies

Targeting: HDAC5 FLJ90614, KIAA0600, NY-CO-9

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Chromatin Immunoprecipitation (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-41117

Provider

Invitrogen Antibodies

Product name

HDAC5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Effects of Manipulation of Noradrenergic Activities on the Expression of Dopaminergic Phenotypes in Aged Rat Brains.

Zeng F, Fan Y, Brown RW, Drew Gill W, Price JB, Jones TC, Zhu MY
ASN neuro 2021 Jan-Dec;13:17590914211055064

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HDAC-5 in NIH 3T3 cell lysate using a HDAC5 polyclonal antibody (Product # PA1-41117) at 2 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HDAC5 in NIH 3T3 cell lysate. Samples were incubated in HDAC5 polyclonal antibody (Product # PA1-41117) using a dilution of 2 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on modified whole cell extracts (1% SDS) of Neuro-2a (Lane 1), U-87 MG (Lane 2), COS-7 (Lane 3), HEK-293 (Lane 4), Jurkat (Lane 5), C2C12 (Lane 6) and NIH/3T3 (Lane 7). The blot was probed with Anti-HDAC5 Polyclonal Antibody (Product # PA1-41117, 2 µg/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 112 kDa band corresponding to HDAC5 was observed across the cell lines tested.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 9. Identify the binding of the HAT & HDAC to the TH promoter by ChIP assay (all N = 4/group). Exposure of MN9D cells to NE for two days increased CBP/p300 binding to TH promoter (A) and reduced the binding of HDAC2 (B) and HDAC5 (C) to the TH promoter. ""Input"" serves as a loading control and rabbit IgG immunoprecipitation serves as a negative control. Top panel in each figures showed the binding to the TH promoter in response to NE treatment. Low panel in each figure showed quantitative real-time PCR of the TH promoter regions from immunoprecipitation with antibody against CBP/p300, HDAC2, and HDAC5. The fold enrichment value is shown as the normalized ChIP signals divided by the normalized input signal. Each bar from both pictures represent data obtained from four separate experiments ( N = 4/group). ** p < .01, compared to the control. NE: treatment with 300 nM NE. Abbreviations: ChIP: chromatin immunoprecipitation assay; HAT: histone acetyl transferase; HDAC: histone deacetylases; NE: norepinephrine; PCR: polymerase chain reaction; TH: tyrosine hydroxylase.