Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AM06769PU-N - Provider product page
- Provider
- OriGene
- Product name
- EIF4B mouse monoclonal antibody, clone 1F5, Purified
- Antibody type
- Monoclonal
- Description
- EIF4B mouse monoclonal antibody, clone 1F5, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- EIF4B
- Isotype
- IgG
- Antibody clone number
- 1F5
- Vial size
- 100 µg
- Concentration
- 1.0 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis using EIF4B antibody against human EIF4B (AA: 381-585) recombinant protein. (Expected MW is 48.3 kDa)
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis using EIF4B antibody against A549 (Lane 1), A431 (Lane 2), HepG2 (Lane 3), HEK293 (Lane 4), HeLa (Lane 5), Jurkat (Lane 6), K562 (Lane 7), NIH3T3 (Lane 8), and MCF-7 (Lane 9) cell lysate.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Red: Control Antigen (100 ng) Purple: Antigen (10 ng) Green: Antigen (50 ng) Blue: Antigen (100 ng)
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NIH3T3 cells using EIF4B antibbody EIF4B (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using EIF4B antibody (green) and Negative Control (red).
- Validation comment
- FC
