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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-92449 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- eIF4B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SQSTRAASIF GGAKPVDTAA REREVEERLQ KEQEKLQRQL DEPKLERRPR ERHPSWRSEE TQERERSRTG SESSQTGTST TSSRNARRRE SEKSLENETL NKEEDCHSPT SKPPKPDQPL KVMPAPPPKE NAWVKRSSNP PARSQSSDTE QQSPTSGGGK VAPAQPSEEG PGRKDENKVD GMNAPKGQTG NSSRGPGDGG NRDHWKESDR KDGKKDQDSR SAPEPKKPEE NPASKFSSAS KYAALSVDGE DENEGEDYAE; Positive Samples: 22Rv1, HeLa, 293T, M21
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.27 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using EIF4B Polyclonal antibody (Product # PA5-92449) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of eIF4B in extracts of various cell lines using eIF4B Polyclonal Antibody (Product # PA5-92449) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of eIF4B was performed in A549 cells using eIF4B Polyclonal Antibody (Product # PA5-92449).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of eIF4B in paraffin-embedded human liver cancer using eIF4B Polyclonal Antibody (Product # PA5-92449) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of eIF4B in paraffin-embedded human prostate using eIF4B Polyclonal Antibody (Product # PA5-92449) at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of eIF4B was performed in 200 µg extracts of 293T cells using eIF4B Polyclonal Antibody (Product # PA5-92449). Western blot was performed from the immunoprecipitate using eIF4B Polyclonal Antibody.
