GTX19389

antibody from GeneTex

Targeting: ERBB3 HER3, LCCS2

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX19389

Provider

GeneTex

Proper citation

GeneTex Cat#GTX19389, RRID:AB_423561

Product name

Her3 / ErbB3 antibody [RTJ2]

Antibody type

Monoclonal

Reactivity

Human, Mouse

Host

Mouse

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Flow cytometry analysis of ErbB3 in HeLa cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ErbB3 antibody [RTJ2] at a dilution of 0.5 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a proper secondary antibody and re-suspended in PBS for FACS analysis.