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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PAB3037 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3037, RRID:AB_1574747
- Product name
- ERBB3 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of ERBB3.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Bone morphogenetic proteins regulate enteric gliogenesis by modulating ErbB3 signaling.
c-erbB3 gene encodes secreted as well as transmembrane receptor tyrosine kinase.
Molecular cloning and expression of an additional epidermal growth factor receptor-related gene.
Isolation and characterization of ERBB3, a third member of the ERBB/epidermal growth factor receptor family: evidence for overexpression in a subset of human mammary tumors.
Chalazonitis A, D'Autréaux F, Pham TD, Kessler JA, Gershon MD
Developmental biology 2011 Feb 1;350(1):64-79
Developmental biology 2011 Feb 1;350(1):64-79
c-erbB3 gene encodes secreted as well as transmembrane receptor tyrosine kinase.
Katoh M, Yazaki Y, Sugimura T, Terada M
Biochemical and biophysical research communications 1993 May 14;192(3):1189-97
Biochemical and biophysical research communications 1993 May 14;192(3):1189-97
Molecular cloning and expression of an additional epidermal growth factor receptor-related gene.
Plowman GD, Whitney GS, Neubauer MG, Green JM, McDonald VL, Todaro GJ, Shoyab M
Proceedings of the National Academy of Sciences of the United States of America 1990 Jul;87(13):4905-9
Proceedings of the National Academy of Sciences of the United States of America 1990 Jul;87(13):4905-9
Isolation and characterization of ERBB3, a third member of the ERBB/epidermal growth factor receptor family: evidence for overexpression in a subset of human mammary tumors.
Kraus MH, Issing W, Miki T, Popescu NC, Aaronson SA
Proceedings of the National Academy of Sciences of the United States of America 1989 Dec;86(23):9193-7
Proceedings of the National Academy of Sciences of the United States of America 1989 Dec;86(23):9193-7
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of ERBB3 polyclonal antibody (Cat # PAB3037) in mouse brain lysate. ERBB3 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with ERBB3 polyclonal antibody (Cat # PAB3037) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of 293 cells using ERBB3 polyclonal antibody (Cat # PAB3037)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
