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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 200-401-205 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-401-205, RRID:AB_2231276
- Product name
- Anti-HER3 (RABBIT) Antibody - 200-401-205
- Antibody type
- Polyclonal
- Vial size
- 250 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Combined immunoprecipitation and western blot of a human cell lysate using anti-HER3 antibody. Lysates were prepared from a human LNCap prostate cell line both with (+) treatment with Heregulin-b1 for 15' at 100 ng/ml and without (-) the addition of Heregulin-b1. The combination of immunoprecipitation and western blotting was performing using 5 µl of the anti-HER3 antibody for immunoprecipitation followed by western blot detection using an anti-phosphotyrosine antibody. Visualization occurred using an ECL system. Film exposure approximately 1. Other detection systems will yield similar results.
- Validation comment
- Western Blot
