GTX105661

antibody from GeneTex

Targeting: BCL2L1 Bcl-X, bcl-xL, bcl-xS, BCL2L, BCLX, PPP1R52

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX105661

Provider

GeneTex

Proper citation

GeneTex Cat#GTX105661, RRID:AB_1949733

Product name

Bcl-X antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

Artocarpin induces cell apoptosis in human osteosarcoma cells through endoplasmic reticulum stress and reactive oxygen species.

Lee CW, Chi MC, Chang TM, Liu JF
Journal of cellular physiology 2019 Aug;234(8):13157-13168

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Benzyl isothiocyanate (BITC) triggers mitochondria-mediated apoptotic machinery in human cisplatin-resistant oral cancer CAR cells.

Lee CF, Chiang NN, Lu YH, Huang YS, Yang JS, Tsai SC, Lu CC, Chen FA
BioMedicine 2018 Sep;8(3):15

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The miR-204-3p-targeted IGFBP2 pathway is involved in xanthohumol-induced glioma cell apoptotic death.

Chen PH, Chang CK, Shih CM, Cheng CH, Lin CW, Lee CC, Liu AJ, Ho KH, Chen KC
Neuropharmacology 2016 Nov;110(Pt A):362-375

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Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent and mitochondrial pathways in mammalian hepatic stellate cells.

Chen MF, Huang SJ, Huang CC, Liu PS, Lin KI, Liu CW, Hsieh WC, Shiu LY, Chen CH
BMC cancer 2016 Jul 26;16:532

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Sam68/KHDRBS1 is critical for colon tumorigenesis by regulating genotoxic stress-induced NF-κB activation.

Fu K, Sun X, Wier EM, Hodgson A, Liu Y, Sears CL, Wan F
eLife 2016 Jul 25;5

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Resveratrol alleviates the cytotoxicity induced by the radiocontrast agent, ioxitalamate, by reducing the production of reactive oxygen species in HK-2 human renal proximal tubule epithelial cells in vitro.

Huang YT, Chen YY, Lai YH, Cheng CC, Lin TC, Su YS, Liu CH, Lai PC
International journal of molecular medicine 2016 Jan;37(1):83-91

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Sam68/KHDRBS1-dependent NF-κB activation confers radioprotection to the colon epithelium in γ-irradiated mice.

Fu K, Sun X, Wier EM, Hodgson A, Hobbs RP, Wan F
eLife 2016 Dec 20;5

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Caspase-3 cleaved p65 fragment dampens NF-κB-mediated anti-apoptotic transcription by interfering with the p65/RPS3 interaction.

Wier EM, Fu K, Hodgson A, Sun X, Wan F
FEBS letters 2015 Nov 30;589(23):3581-7

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An alternative splicing network links cell-cycle control to apoptosis.

Moore MJ, Wang Q, Kennedy CJ, Silver PA
Cell 2010 Aug 20;142(4):625-36

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ?g of whole cell lysate) A:NIH-3T312% SDS PAGE GTX105661 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ?g of whole cell lysate) A: HCT116 12% SDS PAGE GTX105661 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Bcl-X antibody detects BCL2L1 protein by western blot analysis.A. 30 ?g PC-12 whole cell lysate/extract12% SDS-PAGEBcl-X antibody (GTX105661) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence analysis of methanol-fixed A549, using BCL-x(GTX105661) antibody at 1:500 dilution.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Bcl-X antibody detects Bcl-X protein at cytoplasm by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Bcl-X protein stained by Bcl-X antibody (GTX105661) diluted at 1:200.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Hoechst 33342 staining.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunoprecipitation of Bcl-X protein from HCT-116 whole cell extracts using 5 £gg of Bcl-X antibody (GTX105661).Western blot analysis was performed using Bcl-X antibody (GTX105661).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using BCL2L1(GTX105661) antibody at 1:500 dilution.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Bcl-X antibody detects Bcl-X protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Bcl-X antibody (GTX105661) diluted at 1:500.