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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- GTX124266 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX124266, RRID:AB_11172424
- Product name
- Bcl-XS antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Rat
- Host
- Rabbit
Submitted references A high-throughput pipeline for validation of antibodies.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Bcl-XS antibody detects Bcl-XS protein by western blot analysis.A. 30 £gg A549 whole cell lysate/extract B. 30 £gg H1299 whole cell lysate/extract C. 30 £gg HCT116 whole cell lysate/extract D. 30 £gg MCF-7 whole cell lysate/extract 12 % SDS-PAGEBcl-XS antibody (GTX124266) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 £gg) were separated by 15% SDS-PAGE, and the membrane was blotted with Bcl-XS antibody (GTX124266) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Bcl-XS antibody detects Bcl-XS protein at cytosol on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. Bcl-XS antibody (GTX124266) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Bcl-XS antibody detects Bcl-XS protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. Bcl-XS antibody (GTX124266) dilution: 1:500.
