Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- LS-C343999 - Provider product page
- Provider
- LSBio
- Product name
- CTNNA1 / Catenin Alpha-1 Antibody (aa143-292) LS-C343999
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Storage
- At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- CTNNA1 antibody Western blot. All lanes: Anti CTNNA1 at 0.5 ug/ml. WB: Human Recombinant CTNNA1 Protein 0.5ng. Predicted band size: 47 kD. Observed band size: 47 kD.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis of CTNNA1 using anti-CTNNA1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Liver Tissue Lysate Lane 2: Rat Lung Tissue Lysate Lane 3: Rat Cardiac Muscle Tissue Lysate Lane 4: NIH3T3 Whole Cell Lysate Lane 5: PC-12 Whole Cell Lysate Lane 6: HEPG2 Whole Cell Lysate Lane 7: HELA Whole Cell Lysate Lane 8: MCF-7 Whole Cell Lysate Lane 9: HEPA Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNNA1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CTNNA1 at approximately 100KD. The expected band size for CTNNA1 is at 100KD.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- CTNNA1 antibody IHC-paraffin: Rat Intestine Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- CTNNA1 antibody IHC-paraffin: Mouse Intestine Tissue.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- CTNNA1 antibody IHC-paraffin: Human Mammary Tissue.