SM5145P

antibody from Acris Antibodies GmbH

Targeting: CTNNA1 CAP102

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

SM5145P

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#SM5145P, RRID:AB_973863

Product name

anti Catenin alpha-1 / CTNNA1

Antibody type

Monoclonal

Antigen

90 kDa MBP/alpha catenin fusion protein purified on amylose column

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1G5

Vial size

0.1 mg

Concentration

1.0 mg/ml

References

Submitted references

N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane.

Wahl JK 3rd, Kim YJ, Cullen JM, Johnson KR, Wheelock MJ
The Journal of biological chemistry 2003 May 9;278(19):17269-76

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Characterization of cadherin-24, a novel alternatively spliced type II cadherin.

Katafiasz BJ, Nieman MT, Wheelock MJ, Johnson KR
The Journal of biological chemistry 2003 Jul 25;278(30):27513-9

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Mechanism of extracellular domain-deleted dominant negative cadherins.

Nieman MT, Kim JB, Johnson KR, Wheelock MJ
Journal of cell science 1999 May;112 ( Pt 10):1621-32

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Expression of N-cadherin by human squamous carcinoma cells induces a scattered fibroblastic phenotype with disrupted cell-cell adhesion.

Islam S, Carey TE, Wolf GT, Wheelock MJ, Johnson KR
The Journal of cell biology 1996 Dec;135(6 Pt 1):1643-54

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Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin.

Knudsen KA, Soler AP, Johnson KR, Wheelock MJ
The Journal of cell biology 1995 Jul;130(1):67-77

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Identification of plakoglobin domains required for association with N-cadherin and alpha-catenin.

Sacco PA, McGranahan TM, Wheelock MJ, Johnson KR
The Journal of biological chemistry 1995 Aug 25;270(34):20201-6

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunofluorescent analysis of alpha Catenin using Catenin alpha Monoclonal Antibody (1G5) (Cat.-No SM5145P) shows staining in Hela Cells. Catenin alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Catenin alpha (Cat.-No SM5145P) at a dilution of 1:20 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunofluorescent analysis of alpha Catenin using Catenin alpha Monoclonal Antibody (1G5) (Cat.-No SM5145P) shows staining in A2058 Cells. Catenin alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Catenin alpha (Cat.-No SM5145P) at a dilution of 1/20 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.