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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- V3202 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Alpha Catenin Antibody
- Antibody type
- Monoclonal
- Description
- Purified
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 1G5
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)
- Concentration
- 0.2 mg/ml
- Storage
- Store the Alpha Catenin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Slug (SNAI2) expression in oral SCC cells results in altered cell-cell adhesion and increased motility.
GPS autoproteolysis is required for CD97 to up-regulate the expression of N-cadherin that promotes homotypic cell-cell aggregation.
Clarin-1, encoded by the Usher Syndrome III causative gene, forms a membranous microdomain: possible role of clarin-1 in organizing the actin cytoskeleton.
E-cadherin/catenin complexes are formed cotranslationally in the endoplasmic reticulum/Golgi compartments.
Alpha(E)-Catenin induces SRF-dependent transcriptional activity through its C-terminal region and is partly RhoA/ROCK-dependent.
Expression of inappropriate cadherins by epithelial tumor cells promotes endocytosis and degradation of E-cadherin via competition for p120(ctn).
Cadherin and catenin expression in normal human bronchial epithelium and non-small cell lung cancer.
VE-cadherin and desmoplakin are assembled into dermal microvascular endothelial intercellular junctions: a pivotal role for plakoglobin in the recruitment of desmoplakin to intercellular junctions.
Protein kinase C activation upregulates intercellular adhesion of alpha-catenin-negative human colon cancer cell variants via induction of desmosomes.
Characterization of the interactions of alpha-catenin with alpha-actinin and beta-catenin/plakoglobin.
The tyrosine kinase substrate p120cas binds directly to E-cadherin but not to the adenomatous polyposis coli protein or alpha-catenin.
Chromosome 5 suppresses tumorigenicity of PC3 prostate cancer cells: correlation with re-expression of alpha-catenin and restoration of E-cadherin function.
Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin.
Katafiasz D, Smith LM, Wahl JK 3rd
Cell adhesion & migration 2011 Jul-Aug;5(4):315-22
Cell adhesion & migration 2011 Jul-Aug;5(4):315-22
GPS autoproteolysis is required for CD97 to up-regulate the expression of N-cadherin that promotes homotypic cell-cell aggregation.
Hsiao CC, Chen HY, Chang GW, Lin HH
FEBS letters 2011 Jan 21;585(2):313-8
FEBS letters 2011 Jan 21;585(2):313-8
Clarin-1, encoded by the Usher Syndrome III causative gene, forms a membranous microdomain: possible role of clarin-1 in organizing the actin cytoskeleton.
Tian G, Zhou Y, Hajkova D, Miyagi M, Dinculescu A, Hauswirth WW, Palczewski K, Geng R, Alagramam KN, Isosomppi J, Sankila EM, Flannery JG, Imanishi Y
The Journal of biological chemistry 2009 Jul 10;284(28):18980-93
The Journal of biological chemistry 2009 Jul 10;284(28):18980-93
E-cadherin/catenin complexes are formed cotranslationally in the endoplasmic reticulum/Golgi compartments.
Curtis MW, Johnson KR, Wheelock MJ
Cell communication & adhesion 2008 Nov;15(4):365-78
Cell communication & adhesion 2008 Nov;15(4):365-78
Alpha(E)-Catenin induces SRF-dependent transcriptional activity through its C-terminal region and is partly RhoA/ROCK-dependent.
Merdek KD, Jaffe AB, Dutt P, Olson MF, Hall A, Fanburg BL, Kayyali US, Toksoz D
Biochemical and biophysical research communications 2008 Feb 15;366(3):717-23
Biochemical and biophysical research communications 2008 Feb 15;366(3):717-23
Expression of inappropriate cadherins by epithelial tumor cells promotes endocytosis and degradation of E-cadherin via competition for p120(ctn).
Maeda M, Johnson E, Mandal SH, Lawson KR, Keim SA, Svoboda RA, Caplan S, Wahl JK 3rd, Wheelock MJ, Johnson KR
Oncogene 2006 Aug 3;25(33):4595-604
Oncogene 2006 Aug 3;25(33):4595-604
Cadherin and catenin expression in normal human bronchial epithelium and non-small cell lung cancer.
Smythe WR, Williams JP, Wheelock MJ, Johnson KR, Kaiser LR, Albelda SM
Lung cancer (Amsterdam, Netherlands) 1999 Jun;24(3):157-68
Lung cancer (Amsterdam, Netherlands) 1999 Jun;24(3):157-68
VE-cadherin and desmoplakin are assembled into dermal microvascular endothelial intercellular junctions: a pivotal role for plakoglobin in the recruitment of desmoplakin to intercellular junctions.
Kowalczyk AP, Navarro P, Dejana E, Bornslaeger EA, Green KJ, Kopp DS, Borgwardt JE
Journal of cell science 1998 Oct;111 ( Pt 20):3045-57
Journal of cell science 1998 Oct;111 ( Pt 20):3045-57
Protein kinase C activation upregulates intercellular adhesion of alpha-catenin-negative human colon cancer cell variants via induction of desmosomes.
van Hengel J, Gohon L, Bruyneel E, Vermeulen S, Cornelissen M, Mareel M, von Roy F
The Journal of cell biology 1997 Jun 2;137(5):1103-16
The Journal of cell biology 1997 Jun 2;137(5):1103-16
Characterization of the interactions of alpha-catenin with alpha-actinin and beta-catenin/plakoglobin.
Nieset JE, Redfield AR, Jin F, Knudsen KA, Johnson KR, Wheelock MJ
Journal of cell science 1997 Apr;110 ( Pt 8):1013-22
Journal of cell science 1997 Apr;110 ( Pt 8):1013-22
The tyrosine kinase substrate p120cas binds directly to E-cadherin but not to the adenomatous polyposis coli protein or alpha-catenin.
Daniel JM, Reynolds AB
Molecular and cellular biology 1995 Sep;15(9):4819-24
Molecular and cellular biology 1995 Sep;15(9):4819-24
Chromosome 5 suppresses tumorigenicity of PC3 prostate cancer cells: correlation with re-expression of alpha-catenin and restoration of E-cadherin function.
Ewing CM, Ru N, Morton RA, Robinson JC, Wheelock MJ, Johnson KR, Barrett JC, Isaacs WB
Cancer research 1995 Nov 1;55(21):4813-7
Cancer research 1995 Nov 1;55(21):4813-7
Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin.
Knudsen KA, Soler AP, Johnson KR, Wheelock MJ
The Journal of cell biology 1995 Jul;130(1):67-77
The Journal of cell biology 1995 Jul;130(1):67-77
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human A431 cell lysate with Alpha Catenin antibody (clone 1G5). Expected molecular weight ~102 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) A431 and 2) MCF7 cell lysate with Alpha Catenin antibody (clone 1G5). Expected molecular weight ~102 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free Alpha Catenin Antibody (clone 1G5). Confirmation of Integrity and Purity of the Antibody.
